View Vacancy -- Postdoctoral Scientist - PNAC - Dr Patrycja Kozik - LMB 2780

The MRC Laboratory of Molecular Biology (LMB) is one of the birthplaces of modern molecular biology and has played an important role in the development of many new techniques, most notably protein crystallography, cryo-electron microscopy, DNA sequencing and monoclonal antibodies. The LMB has a clear goal of understanding biological processes at the molecular level, with the ultimate aim of using this knowledge to tackle specific problems in human health and disease. We aim to tackle difficult, long-term problems, which often require investment over many years.

There are four scientific Divisions - Structural Studies, Protein and Nucleic Acid Chemistry, Cell Biology and Neurobiology. However, scientific collaboration between the different Divisions is extensive. Scientists are well supported by excellent scientific facilities and by the LMB Operations Group which maintains the core infrastructure and services of the institute.

The LMB provides an unsurpassed environment for both new and established researchers. Scientists are drawn to the LMB from all over the world, thus creating a lively and international community for the exchange of ideas and technical innovation.

Overall purpose: 

To work within the group of Dr. Patrycja Kozik at the MRC Laboratory of Molecular Biology (LMB), within a programme aimed at investigating initiation immune responses by dendritic cells. Specifically, to drive a project that employs targeted protein degradation in order to facilitate T cell-mediated immune responses.

The successful candidate will use chemical and biological degraders to channel proteins for proteasomal degradation, and with a goal of enhancing both presentation of endogenous antigens in cancer cells as well as antigen cross-presentation in DCs. The project will be performed in collaboration with Dr. Abbie Macmillan-Jones in the Discovery Sciences Group at AstraZeneca.

Main duties:

•    To undertake research aimed at enhancing cytosolic degradation of antigens destined for presentation on MHC class I.
•    To identify, develop and apply a broad range of techniques to pursue the research objectives.
•    To present scientific work at seminars within the laboratory and at external meetings.
•    To contribute to laboratory-wide discussions on developments within the field. 
•    To draft scientific papers, and contribute to the overall preparation of research for publication.
•    To contribute to the LMB’s mission in the public engagement of science, and the translation of research findings into improvements in health care.
•    To assist in the training of PhD students and other members of the LMB.

Key responsibilities:

Within the overall direction of the programme, the group, the remit of the project and in discussion with the Group Leader, you will make a significant input into determining the direction of the project within a two year lifespan. 

To plan your own work and objectives on a 12-month basis and manage your experimental work within the project along with your Supervisor.

To work with limited supervision to identify, develop, modify and apply the necessary techniques to achieve the goals of the project.

To introduce and apply new techniques across a wide range of disciplines and to have the creativity and initiative to develop novel approaches and methods where required.

To ensure the research is carried out in accordance with good practice and in compliance with local policies and legal requirements.

To contribute to the smooth running of the group, including the effective use of resources, training of others and taking responsibility for use of communal facilities.

To enhance your research and generic skills through a tailored development programme. 

Working relationships: 

You will report to Dr Kozik and will interact and collaborate with other Postdoctoral Scientists, Research Support staff and students, not only in your group, but also across the LMB and with external groups as necessary.

Additional information:

This is a two-year training and development position for a Postdoctoral Scientist who has recently completed their doctoral studies, is moving into a new research discipline or has limited experience of key transferable skills. We support Postdoctoral Scientists with a range of formal and on-the-job training, including:
•    MRC training courses.
•    External training and personal development courses.
•    One-to-one training with your Supervisor and other Scientists.

You will commit to undertaking the following:
•    Developing and following a personal development plan.
•    Attending training courses.
•    Identifying additional training which will support you to develop your career.

Academic qualifications: 

These should include a PhD in a relevant subject or due to complete PhD within 6 months.

Technical skills and expertise:

Expertise in molecular mechanisms involved in cytosolic degradation of proteins OR antigen presentation on MHC class I. 

Solid knowledge of dendritic cell biology OR T cell biology.

Experience of and ability to perform techniques relevant to the project.  Particularly useful would be experience of: 
-    Construct design and molecular biology techniques
-    Extensive experience culturing immune cells (dendritic cells and/or T cells).
-    Strong expertise in flow cytometry

Desirable:
-    Experience with analysis of protein degradation efficiency
-    Experience monitoring efficiency of antigen presentation or T cell priming
-    Ability to perform in vitro transcription reaction (IVT) 
-    Experience with vaccination experiments in mice

Track record of research:

This will include impactful contributions to scientific research and/or methods development. 

Other relevant evidence of: 

•    Commitment.
•    Originality.
•    Ability to communicate.
•    Working with others.

Additional information: 

The position would suit an individual who enjoys working in a collaborative environment, manages their time effectively and is motivated by setting and reaching project milestones.

You must at all times carry out your responsibilities with due regard to the UKRI: 
•    Code of Conduct
•    Equality, Diversity and Inclusion policy
•    Health and Safety policy
•    Data Protection policy

Job descriptions should be reviewed on a regular basis and at the annual appraisal. Any changes should be made and agreed between you and your manager.

The above lists are not exhaustive and you are required to undertake such duties as may reasonably be requested within the scope of the post. All employees are required to act professionally, co-operatively and flexibly in line with the requirements of the post, the MRC and UKRI.  

The role holder will be required to have the appropriate level of security screening/vetting required for the role. UKRI reserves the right to run or re-run security clearance as required during the course of employment.