View Vacancy -- Research Support Officer - Structural Studies - Dr Matteo Allegretti - LMB 2356

Open Date
15/02/2024, 13:00
Close Date
18/03/2024, 23:55
MRC Laboratory of Molecular Biology

The MRC Laboratory of Molecular Biology (LMB) is one of the birthplaces of modern molecular biology and has played an important role in the development of many new techniques, most notably protein crystallography, cryo-electron microscopy, DNA sequencing and monoclonal antibodies. The LMB has a clear goal of understanding biological processes at the molecular level, with the ultimate aim of using this knowledge to tackle specific problems in human health and disease. We aim to tackle difficult, long-term problems, which often require investment over many years.

There are four scientific Divisions - Structural Studies, Protein and Nucleic Acid Chemistry, Cell Biology and Neurobiology. However, scientific collaboration between the different Divisions is extensive. Scientists are well supported by excellent scientific facilities and by the LMB Operations Group which maintains the core infrastructure and services of the institute.

The LMB provides an unsurpassed environment for both new and established researchers. Scientists are drawn to the LMB from all over the world, thus creating a lively and international community for the exchange of ideas and technical innovation.

UK Research and Innovation is a new entity that brings together nine partners to create an independent organisation with a strong voice for research and innovation, more information can be found at www.ukri.org

MRC - 5
Cambridge
£29,808 - £34,085 per annum
Fixed Term
Science
Full Time
3 years

Overall purpose: 

To work for Dr Alia dos Santos, hosted by the group of Dr Matteo Allegretti, at the Laboratory of Molecular Biology (LMB). This is a supportive research role funded by the Wellcome Trust, to investigate how nuclear envelope repair occurs in mammalian cells. The project takes an interdisciplinary approach that includes the use of cryo-electron tomography for in situ structural biology.

Main duties/ Key responsibilities:

•    To assist in research aimed at understanding nuclear envelope repair.
•    Maintain mammalian cell lines and help in the creation of stable cell lines.
•    To perform molecular biology, cloning, pull-downs and biochemical assays, under the guidance of your supervisor.
•    To assist in sample preparation and data analysis for fluorescence microscopy, including super-resolution microscopy.
•    To assist in protein expression and purification assays.
•    Keep detailed and accurate records.
•    To ensure the research is carried out in accordance with good practice and in compliance with local policies and legal requirements.
•    To plan and manage your experimental work according to the objectives set by your supervisor.
•    To assist in sample preparation for cryo-electron tomography and support the development of new methods for in situ structural biology.
•    To support cryo-electron microscopy data analysis.
•    To actively develop and troubleshoot challenging assays.
•    To assist in maintaining general lab stocks and reagents.
•    To contribute to the smooth running of the group, including the effective use of resources and taking responsibility for use of communal facilities.

Working relationships: 

You will report to Dr dos Santos and Dr Allegretti, and you will interact with other members of the group and also with other scientists at the LMB as necessary.

Additional information:

The above lists are not exhaustive and you are required to undertake such duties as may reasonably be requested within the scope of the post.  All employees are required to act professionally, co-operatively and flexibly in line with the requirements of the post and the MRC.

Education / qualifications / training required: 

Essential: Bachelor’s degree in Biochemistry, Cell or Molecular Biology or a related subject. Experience of lab work covering the technical skills listed below

Desirable: Research Master’s degree in a related biological subject.

Previous work experience required/ technical skills: 

Essential: 
•    Mastery of aseptic technique for mammalian cell culture, including passaging and transfections.
•    Experience in construct design and cloning.
•    Able to perform biochemical assays, SDS-PAGE, Western Blot, bacterial transformations, and plasmid DNA purification.
•    Previous experience in optical light microscopy, including confocal.

Desirable: 
•    Experience in Immunocytochemistry.
•    Knowledge of CRISPR methods for gene modification.
•    Knowledge of bioinformatics tools (Ensembl, SnapGene, BLAST, etc.)
•    Programming skills in Python

Knowledge and experience: 

Independent working

Essential: 
•    The ability to work safely without close supervision.
•    The ability to follow complex protocols.
•    Good communication skills to allow accurate information to be exchanged.

Desirable: 
•    Able to acquire new techniques quickly and independently.

Personal skills / behaviours / qualities: 

Essential: 
•    Highly organised and detail-oriented individual.
•    Willingness to independently develop, troubleshoot, and validate new methods or pipelines.
•    Theoretical and/or practical training in genetics or biology.
•    A mature and conscientious attitude.
•    Skills and experience in working collaboratively as a member of a team.

Desirable:
•    Proactively try new methods.

Corporate/Local responsibilities & requirements

You must at all times carry out your responsibilities with due regard to the UKRI: 
•    Code of Conduct
•    Equality, Diversity and Inclusion policy
•    Health and Safety policy
•    Data Protection policy

Job descriptions should be reviewed on a regular basis and at the annual appraisal.  Any changes should be made and agreed between you and your manager.

The above lists are not exhaustive and you are required to undertake such duties as may reasonably be requested within the scope of the post.  All employees are required to act professionally, co-operatively and flexibly in line with the requirements of the post, the MRC and UKRI.  

The role holder will be required to have the appropriate level of security screening/vetting required for the role. UKRI reserves the right to run or re-run security clearance as required during the course of employment.

The MRC is a great place to work and progress your career, be it in scientific research or the support functions.The MRC is a unique working environment where our researchers are rewarded by world class innovation and collaboration opportunities that the MRC name brings. The MRC is an excellent place to develop yourself further and a range of training & development opportunities will be available to you, including professional registration with the Science Council.

Choosing to come to work at the MRC (part of UKRI) means that you will have access to a whole host of benefits from a defined benefit pension scheme and excellent holiday entitlement to access to employee shopping/travel discounts and salary sacrifice cycle to work scheme, as well as the chance to put the MRC and UKRI on your CV in the future.

Our success is dependent upon our ability to embrace diversity and draw on the skills, understanding and experience of all our people. We welcome applications from all sections of the community irrespective of gender, race, ethnic or national origin, religion or belief, sexual orientation, disability or age. As "Disability Confident" employers, we guarantee to interview all applicants with disabilities who meet the minimum criteria for the vacancy.

UKRI supports research in areas that include animal health, agriculture and food security, and bioscience for health which includes research on animals, genetic modification and stem cell research. Whilst you may not have direct involvement in this type of research, you should consider whether this conflicts with your personal values or beliefs.

We will conduct a full and comprehensive pre-employment check as an essential part of the recruitment process on all individuals that are offered a position with UKRI. This will include a security check and an extreme organisations affiliation check.  The role holder will be required to have the appropriate level of security screening/vetting required for the role.  UKRI reserves the right to run or re-run security clearance as required during the course of employment.

This opportunity is closed to applications.