View Vacancy -- Research Support - PNAC - Dr Andrew McKenzie - LMB 1072
The MRC Laboratory of Molecular Biology (LMB) is one of the birthplaces of modern molecular biology and has played an important role in the development of many new techniques, most notably protein crystallography, cryo-electron microscopy, DNA sequencing and monoclonal antibodies. The LMB has a clear goal of understanding biological processes at the molecular level, with the ultimate aim of using this knowledge to tackle specific problems in human health and disease. We aim to tackle difficult, long-term problems, which often require investment over many years.
There are four scientific Divisions - Structural Studies, Protein and Nucleic Acid Chemistry, Cell Biology and Neurobiology. However, scientific collaboration between the different Divisions is extensive. Scientists are well supported by excellent scientific facilities and by the LMB Operations Group which maintains the core infrastructure and services of the institute.
The LMB provides an unsurpassed environment for both new and established researchers. Scientists are drawn to the LMB from all over the world, thus creating a lively and international community for the exchange of ideas and technical innovation.
To work within the group of Dr Andrew McKenzie at the Laboratory of Molecular Biology (LMB), providing research support for a programme investigating type-2 immunity in health and disease.
• To undertake basic research by preparing, setting up, conducting and recording the outcome of experiments, analysing and interpreting results as appropriate.
• To perform a range of experiments that may include some but not necessarily all of:
o Design, reagent preparation, propagation and characterization of knockout cell lines using CRISPR/Cas9 technology.
o Analysis of experimental mouse models.
o Implement a range of molecular biology procedures required for the generation and analysis of transgenic mice.
o Cell culture assays (eg infection, qPCR, microscopy, immunoprecipitation).
• To present information on research progress and outcomes as appropriate.
• To conduct literature and database searches.
• To contribute to the production of research reports and publications.
• To ensure and maintain an efficient and tidy Laboratory.
To plan and manage your experimental work within the project along with your supervisor and other members of the project team.
To ensure the research is carried in accordance with good practice and in compliance with local polices and legal requirements.
To contribute to the smooth running of the research group, including the effective use of resources, training of others and taking responsibility for use of communal facilities.
You will report to Dr Andrew McKenzie. You will interact and collaborate with Postdoctoral Scientists, Research Support Staff and Students, not only in your Group, but also across the LMB and with external Groups as necessary.
Some flexibility in working hours might be required to keep cells over weekends.
The above lists are not exhaustive and you are required to undertake such duties as may reasonably be requested within the scope of the post. All employees are required to act professionally, co-operatively and flexibly in line with the requirements of the post and the MRC.
Education / qualifications / training required:
Essential: Bachelors degree or equivalent in a relevant biological subject.
Desirable: Relevant Masters or equivalent relevant laboratory experience.
Previous work experience required:
• Basic bench work skills.
• Experience or theoretical knowledge of basic molecular biology skills/techniques (eg PCR, gene cloning).
• Previous experience in the field of immunology or similar.
• Experimental techniques relevant to the role, which could include (though not limited to):
o Molecular biology techniques: PCR, gene cloning, DNA/RNA extraction, primer and probe design, probe synthesis, in situ hybridization.
o Protein analysis: protein production and purification (affinity chromatography), gel electrophoresis, western blot.
o Mouse models.
o Cell culture.
o CRISPR/Cas9 gene targeting system.
o FACS and/or fluorescence microscopy experience.
Knowledge and Experience:
Creativity and initiative
• Ability to plan and carry out experiments, collate and analyse data.
• Understanding of laboratory health and safety.
Desirable: Practical experience of running and maintaining scientific equipment.
Essential: Proven skills in working independently and as part of a team with minimal supervision as well as good judgment in assessing when to ask for support.
Desirable: Can independently advance existing techniques and implement new methods.
Verbal and written communication skills
Essential: Excellent written and verbal communication skills.
• Experience of giving presentations.
• Has made contributions to scientific papers.
Personal skills / behaviours / qualities:
• Methodical and thorough with strong organisational skills.
• Passion and enthusiasm for science and a desire to continue to develop skills and knowledge.
• Proactive in seeking solutions to problems and keen to take responsibility.
Choosing to come to work at the MRC (part of UKRI) means that you will have access to a whole host of benefits from a defined benefit pension scheme and excellent holiday entitlement to access to employee shopping/travel discounts and salary sacrifice cycle to work scheme, as well as the chance to put the MRC and UKRI on your CV in the future.
Our success is dependent upon our ability to embrace diversity and draw on the skills, understanding and experience of all our people. We welcome applications from all sections of the community irrespective of gender, race, ethnic or national origin, religion or belief, sexual orientation, disability or age. As "Disability Confident" employers, we guarantee to interview all applicants with disabilities who meet the minimum criteria for the vacancy.
UKRI supports research in areas that include animal health, agriculture and food security, and bioscience for health which includes research on animals, genetic modification and stem cell research. Whilst you may not have direct involvement in this type of research, you should consider whether this conflicts with your personal values or beliefs.
We will conduct a full and comprehensive pre-employment check as an essential part of the recruitment process on all individuals that are offered a position with UKRI. This will include a security check and an extreme organisations affiliation check.