View Vacancy -- Research Assistant - MLC 1695

Overall purpose:  

The Mary Lyon Centre at the Medical Research Council Harwell requires three Research Assistants to provide technical support in a busy Genome Engineering service team. The successful candidates will work within the Molecular and Cellular Biology (MCB) group and in close collaboration with the Genotyping core facility. Their activity will span a broad range of molecular and cellular biology techniques. 

The available posts are based within established laboratories involved in the generation and quality control of mouse targeted alleles and genotyping. They will work as part of a team and be responsible for performing a range of Molecular Biology techniques (including generation of CRISPR reagents, PCR, Sanger sequencing analysis, digital droplet PCR, non-radioactive Southern blotting and qPCR-based genotyping) and embryonic stem (ES) cell culture. Emphasis on particular techniques will vary according to service demand. 

Previous experience of some of these techniques will be an advantage, but not essential, as training can be provided. Candidates searching for a part time role are also welcome to apply.

Main duties / key responsibilities:

Characterisation and quality control of genetically modified materials employing PCR-based techniques, sequencing and non-radioactive Southern blotting. Genotyping. Sample management and communication of results. Some cell culture. 

Other general duties required are to ensure smooth running of a Molecular Biology laboratory i.e. ordering reagents – tasks shared with rest of team.

Working relationships:

• Process samples/tasks by working as a team with the other Molecular and Cellular Biology (MCB) team members, as directed by the Head of MCB initially or by the External Genotyping Coordinator as per workload demands. 
• Work with scientists in the different research groups, to help process samples as efficiently as possible.

Additional information:

Some flexible work patterns and paid weekend work may be required at the discretion of the supervisor.

Equality & Diversity:

The MRC values the diverse skills and experience of its employees and is committed to achieving equality of treatment for all. Our objectives are that all individuals shall have equal opportunities for employment and advancement on the basis of their skills, aptitudes and abilities. The MRC is committed to the engagement and retention of the best possible talent and to creating an environment that encourages excellence in scientific research through good equalities and diversity leadership and management.

Corporate/Local responsibilities & requirements:

The job holder must at all times carry out their responsibilities with due regard to the MRC’s: 

• Code of Conduct
• Equality and Diversity policy
• Health and Safety policy
• Data Protection and Security policy 

Job descriptions should be reviewed on a regular basis and at the annual appraisal. Any changes should be made and agreed between the post holder and their manager. 

The above lists are not exhaustive and the job holder is required to undertake such duties as may reasonably be requested within the scope of the post. All employees are required to act professionally, co-operatively and flexibly in line with the requirements of the post and the MRC.

Education / Qualifications / Training required:

Essential:

• BSc in Biological Sciences or related. Understanding of basic techniques (qPCR, sequencing) 

Desirable:

• MSc in Biological Sciences with several years laboratory-based molecular biology training. Understanding of genome engineering techniques (gene targeting, CRISPR-based tools)

Previous work experience required:

Essential:

• Work in a laboratory

Desirable:

• Previous work with genetically modified materials
• Previous experience in genotyping would be desirable, although some training in molecular techniques and automated liquid handling can be given
• Evidence of quality and customer focused work

Knowledge and experience:

Essential:

• Experience in basic techniques of Molecular Biology e.g. PCR, cloning, Southern blotting
• Computer literacy is essential for the recording and communication of results
• An understanding of genetic modification in laboratory animals

Desirable:

• Previous experience with DNA sequence analysis software
• Experience of work in sterile conditions
• Working and adhering to standard operating procedures and experience of process analysis and refinement

Personal skills/behaviours/qualities:

Essential:

• Organised, hard-working with a strong eye for detail and is able to work well as part of a team

Desirable:

• Able to communicate clearly and concisely with a variety of scientists at all levels (from Principal investigator to research assistants and animal technicians)