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View Vacancy -- Genome Engineering Specialist - MLC 631

Open Date
17/08/2017 13:55
Close Date
24/09/2017 23:55
MRC Mary Lyon Centre
We are a major international research centre at the forefront of studies in mouse
genetics and functional genomics. We are investigating a wide variety of
disease models in mice, in order to enhance our understanding of the molecular
and genetic basis of disease
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MRC - 4
Didcot, Oxfordshire
£27,629 - ££32,975
Fixed Term
Technical & Scientific Professionals
Full Time
To 31.01.2020

Overall purpose:

The Mary Lyon Centre (MLC) at the Medical Research Council Harwell Institute requires a skilled molecular biologist to support a busy genome engineering service team.

The MLC undertakes generation and quality control of mouse targeted alleles, for both large-scale mutagenesis programmes or as a bespoke service for individual laboratories. The post holder will be based in the Molecular and Cellular Biology group and coordinate the genome engineering work. They will work in a twelve-strong team under the guidance of the Molecular and Cellular Biology Deputy Manager.

Strong molecular biology and organisational skills are essential. Experience in the handling of either CRISPR reagents and RNAs or generation of complex plasmid based-constructs is desirable but not essential, as training can be provided.

Main duties / key responsibilities:

They will be responsible for the design, implementation and quality control of new alleles. In addition they will communicate regularly with MLC’s customers, other relevant MLC departments and present the work of the service in conferences and courses.

Duties include

- The design and generation of genome engineering strategies and reagents, inclusive of synthesis of RNA-based tools.

Quality control of genetically modified materials employing PCR-based and sequencing techniques and non-radioactive Southern blotting. Genotyping. Sample management and communication of results.

Supporting Molecular and Cellular Biology team members with designing and troubleshooting their tasks.

Working relationships:

Creating genetic alterations in the mouse genome as part of the Molecular and Cellular Biology team, under the supervision of the Molecular and Cellular Biology Deputy Manager and with the support of 2 research assistants within a twelve-strong team.

Helps to coordinate work with relevant MLC teams (i.e. GEMS and Gene Delivery team)


Work with scientists in the different research groups requiring new mouse mutants to gather specifications and communicate on progress

Additional information:

Some flexible work patterns and occasional weekend work will be required.


Previous work with genetically modified materials

Previous experience in genotyping

High level for the recording and communication of results

Previous experience with DNA sequence analysis software

Understanding of basic techniques of Molecular Biology e.g. qPCR, Southern blotting

Understanding of genetic modification in laboratory animals

Understanding of methods employed for mouse genome engineering

Organised, hard-working with a strong eye for detail, ability to handle complex information and is able to work well as part of a team

Ability to work independently and prioritise weekly work schedule

Ability to work towards targets for deliverables


PhD in Biological Sciences or related, or equivalent experience

Evidence of quality and customer focused work

Work with RNA

Experience of use of a LIMS

Experience of qPCR/ddPCR application

Experience of mouse genome engineering

Previous work in aseptic conditions

Able to communicate clearly and concisely with a variety of scientists at all levels (from Principal investigator to research assistants and animal technicians)

Experience of method development/refinement

Experience of handling multiple complex datasets


Proven history of efficient time management

Working and adhering to standard operating procedures

The MRC is a great place to work and progress your career, be it in scientific research or the support functions.

The MRC is a unique working environment where our researchers are rewarded by world class innovation and collaboration opportunities that the MRC name brings. The MRC is an excellent place to develop yourself further and a range of training & development opportunities will be available to you, including professional registration with the Science Council.

Choosing to come to work at the MRC (part of UKRI) means that you will have access to a whole host of benefits from a defined benefit pension scheme and excellent holiday entitlement to access to employee shopping/travel discounts and salary sacrifice cycle to work scheme, as well as the chance to put the MRC and UKRI on your CV in the future.

Our success is dependent upon our ability to embrace diversity and draw on the skills, understanding and experience of all our people. We welcome applications from all sections of the community irrespective of gender, race, ethnic or national origin, religion or belief, sexual orientation, disability or age. As "Disability Confident" employers, we guarantee to interview all applicants with disabilities who meet the minimum criteria for the vacancy.

Final appointments will be subject to pre-employment screening.

This opportunity is closed to applications.