View Vacancy -- Postdoctoral Scientist - PNAC - Dr Andrew McKenzie - LMB 724
The MRC Laboratory of Molecular Biology (LMB) is one of the birthplaces of modern molecular biology and has played an important role in the development of many new techniques, most notably X-ray crystallography of proteins, DNA sequencing and monoclonal antibodies. The LMB has a clear goal of understanding biological processes at the molecular level, with the ultimate aim of using this knowledge to tackle specific problems in human health and disease. We aim to tackle difficult, long-term problems, which often require investment over many years.
There are four scientific Divisions - Structural Studies, Protein and Nucleic Acid Chemistry, Cell Biology and Neurobiology. However, scientific collaboration between the different Divisions is extensive. Scientists are well supported by excellent scientific facilities and by the LMB Operations Group which maintains the core infrastructure and services of the institute.
With the new laboratory the LMB provides an unsurpassed environment for both new and established researchers. Scientists are drawn to the LMB from all over the world, thus creating a lively and international community for the exchange of ideas and technical innovation.
To work within the research group of Dr Andrew McKenzie at the MRC Laboratory of Molecular Biology (LMB), within a programme aimed at investigating immune and haematopoetic disorders. Specifically to undertake research to investigate the molecular regulation of innate lymphoid cells (ILC) and their roles in health and disease (see e.g. Cell Rep. (2017) 18, 1893; J Exp Med. (2017) 214, 2507; Nat. Immunol. (2016) 17, 57; J. Exp. Med. (2015) 12, 875; Immunity (2014) 41, 283; Nat. Immunol. (2012) 13, 229).
The ILC family includes ILC1 (IFNg-expressing), ILC2 (IL-5 and IL-13-expressing) and ILC3 (IL-22 and IL-17-expressing cells). Significant roles are emerging for ILCs in protective immunity against parasitic helminths (ILC2) and bacteria (ILC1 and ILC3), and in autoimmune disorders (ILC1 and ILC3) and allergic disease (ILC2). To interrogate the roles of ILCs in protecting mucosal barriers and their functions in potentiating adaptive immunity, we have generated a panel of novel reagents. These gene-reporter and cell-depletion strains will be used to define how ILCs contribute to disease.
• To undertake research aimed at elucidating the molecular and cellular regulation of ILCs.
• To identify, develop and apply a broad range of techniques to pursue the research objectives.
• To present scientific work at seminars within the Laboratory and at external meetings.
• To contribute to Laboratory-wide discussions on developments within the field.
• To draft scientific papers, and contribute to the overall preparation of research for publication.
• To contribute to the LMB’s mission in the public engagement of science, and the translation of research findings into improvements in health care.
• To assist in the training of PhD Students and other members of the LMB.
Within the overall direction of the programme, the Group, the remit of the project and in discussion with the Group Leader, you will make a significant input into determining the direction of the project within a 3 year lifespan.
To plan your own work and objectives on a 12 month basis and manage your experimental work within the project along with your Supervisor.
To work with limited supervision to identify, develop, modify and apply the necessary techniques to achieve the goals of the project.
To introduce and apply new techniques across a wide range of disciplines and to have the creativity and initiative to develop novel approaches and methods where required.
To ensure the research is carried out in accordance with good practice and in compliance with local policies and legal requirements.
To contribute to the smooth running of the Group, including the effective use of resources, training of others and taking responsibility for use of communal facilities.
To enhance your research and generic skills through a tailored development programme.
You will report to Dr Andrew McKenzie and will interact and collaborate with other Postdoctoral Scientists, Research Support Staff and Students, not only in your Group, but also across the LMB and with external Groups as necessary.
This is a three-year training and development position for a Postdoctoral Scientist who has recently completed their doctoral studies, is moving into a new research discipline or has limited experience of key transferable skills. We support Postdoctoral Scientists with a range of formal and on-the-job training, including:
• MRC training courses.
• External training and personal development courses.
• One-to-one training with your Supervisor and other Scientists.
You will commit to undertaking the following:
• Developing and following a personal development plan.
• Attending training courses.
• Identifying additional training which will support you to develop your career.
The above lists are not exhaustive and you are required to undertake such duties as may reasonably be requested within the scope of the post. All employees are required to act professionally, co-operatively and flexibly in line with the requirements of the post and the MRC.
These should include a PhD in a relevant subject or due to complete PhD within 6 months.
Technical skills and expertise:
Experience of and ability to perform techniques relevant to the project. Particularly useful would be proven experience and knowledge of:-
Experience with cell culture techniques including retro/lentiviral transduction of cell lines and primary cells.
• Experience with multi-colour flow cytometry including extracellular and intracellular staining and fluorescent reporter analysis.
• Knowledge of Flow Jo etc.
• Experience of flow sorting and index cell sorting for single cell analysis.
Experience of immune cell phenotyping in humans and mice, in particular categorising type1 / type2 /3 responses e.g. identifying M1/M2, Th1/2/17, ILC1/2/3 populations.
Proficiency in immunohistology and image analysis.
• Proficiency in cloning experience, PCR (including realtime), genotyping, lentiviral plasmid generation.
• Proficiency in the generation and analysis of bulk and single cell RNA-seq libraries.
• Holds a Personal licence.
• Awareness of breeding strategies.
• Experience of tissue isolation and preparation.
• Knowledge of adoptive cell transfers; bone marrow chimeras; in vivo infection models.
Track record of research:
This will include achievements, and contributions to published papers.
Other relevant evidence of:
• Ability to communicate.
• Working with others.
The MRC is a great place to work and progress your career, be it in scientific research or the support functions.
The MRC is a unique working environment where our researchers are rewarded by world class innovation and collaboration opportunities that the MRC name brings. The MRC is an excellent place to develop yourself further and a range of training & development opportunities will be available to you, including professional registration with the Science Council.
Choosing to come to work at the MRC means that you will have access to a whole host of benefits from a final salary pension scheme and excellent holiday entitlement to access to employee shopping/travel discounts and salary sacrifice cycle to work scheme and childcare vouchers, as well as the chance to put the MRC on your CV in the future.
Our success is dependent upon our ability to embrace diversity and draw on the skills, understanding and experience of all our people. We welcome applications from all sections of the community irrespective of gender, race, ethnic or national origin, religion or belief, sexual orientation, disability or age. As "Disability Confident" employers, we guarantee to interview all applicants with disabilities who meet the minimum criteria for the vacancy.
Final appointments will be subject to pre-employment screening.