View Vacancy -- Postdoctoral Scientist - PNAC - Dr Patrycja Kozik - LMB 871
The MRC Laboratory of Molecular Biology (LMB) is one of the birthplaces of modern molecular biology and has played an important role in the development of many new techniques, most notably protein crystallography, cryo-electron microscopy, DNA sequencing and monoclonal antibodies. The LMB has a clear goal of understanding biological processes at the molecular level, with the ultimate aim of using this knowledge to tackle specific problems in human health and disease. We aim to tackle difficult, long-term problems, which often require investment over many years.
There are four scientific Divisions - Structural Studies, Protein and Nucleic Acid Chemistry, Cell Biology and Neurobiology. However, scientific collaboration between the different Divisions is extensive. Scientists are well supported by excellent scientific facilities and by the LMB Operations Group which maintains the core infrastructure and services of the institute.
The LMB provides an unsurpassed environment for both new and established researchers. Scientists are drawn to the LMB from all over the world, thus creating a lively and international community for the exchange of ideas and technical innovation.
To work within the Group of Dr Patrycja Kozik at the MRC Laboratory of Molecular Biology (LMB), within a programme aimed at understanding the molecular mechanisms involved in the initiation of anti-tumour immune responses by dendritic cells. Specifically, to undertake research to identify and characterise factors that control adjuvanticity or immunosuppressive properties of cancer cells. Key approaches will involve CRISPR-Cas9 arrayed screening, flow cytometry, and a variety of immunological assays. The project will be performed in collaboration with Dr Douglas Ross-Thriepland and Dr Philip Rawlins in the Functional Genomics Group at AstraZeneca, where part of the work will be conducted.
• To undertake research aimed at identifying factors that control adjuvanticity or immunosuppressive properties of cancer cells.
• To identify, develop and apply a broad range of techniques to pursue the research objectives.
• To present scientific work at seminars within the Laboratory and at external meetings.
• To contribute to Laboratory-wide discussions on developments within the field.
• To draft scientific papers, and contribute to the overall preparation of research for publication.
• To contribute to the LMB’s mission in the public engagement of science, and the translation of research findings into improvements in health care.
• To assist in the training of PhD Students and other members of the LMB.
Within the overall direction of the programme, the Group, the remit of the project and in discussion with the Group Leader, you will make a significant input into determining the direction of the project within a 2 year lifespan.
To plan your own work and objectives on a 12 month basis and manage your experimental work within the project along with your Supervisor.
To work with limited supervision to identify, develop, modify and apply the necessary techniques to achieve the goals of the project.
To introduce and apply new techniques across a wide range of disciplines and to have the creativity and initiative to develop novel approaches and methods where required.
To ensure the research is carried out in accordance with good practice and in compliance with local policies and legal requirements.
To contribute to the smooth running of the Group, including the effective use of resources, training of others and taking responsibility for use of communal facilities.
To enhance your research and generic skills through a tailored development programme.
You will report to Drs Kozik, Ross-Thriepland and Rawlins and will interact and collaborate with other Postdoctoral Scientists, Research Support Staff and Students, not only in your Group, but also across the LMB, AstraZeneca and with external Groups as necessary. Therefore you will be required to work across both sites.
It is anticipated that the first 9-12 months of the project will be conducted at AstraZeneca (Cambridge Science Park, Milton Road), followed by follow up studies in the Kozik lab at LMB (Cambridge Biomedical Campus, Francis Crick Avenue).
This is a 2-year training and development position for a Postdoctoral Scientist who has recently completed their doctoral studies, is moving into a new research discipline or has limited experience of key transferable skills. You will get the choice upon appointment of undertaking a Career Development Fellowship or the MRC Post-Doctoral Training Scheme. We support Postdoctoral Scientists with a range of formal and on-the-job training, including:
• MRC training courses.
• External training and personal development courses.
• One-to-one training with your Supervisor and other Scientists.
You will commit to undertaking the following:
• Developing and following a personal development plan.
• Attending training courses.
• Identifying additional training which will support you to develop your career.
The above lists are not exhaustive and you are required to undertake such duties as may reasonably be requested within the scope of the post. All employees are required to act professionally, co-operatively and flexibly in line with the requirements of the post and the MRC.
These should include a PhD in a relevant subject (e.g. cancer biology, immunology etc) or due to complete PhD in a relevant subject within 6 months of the application deadline.
Technical skills and expertise:
• Experience of and ability to perform techniques relevant to the project.
• Proven and extensive experience with cell culture and basic molecular biology techniques.
• Strong expertise in flow cytometry and/or functional genomics.
• Experience with culture and characterisation of immune cells.
• Experience with high-throughput flow platforms (e.g. Intellicyt iQue) or automated fluorescence microscopy platforms.
• Experience of functional genomic screening using technologies like CRISPR (arrayed or pooled) and RNAi.
• Practical experience in high content/multivariate data analysis of complex datasets using advanced features in platforms like R, TIBCO Spotfire, Matlab etc.
• Practical experience in multicolour flow cytometry (antibody-based phenotyping, functional assays, etc).
Track record of research:
Evidence of impactful contributions to scientific research and/or methods development, and ability to work effectively within a team or collaborative group.
Other relevant evidence of:
• Ability to communicate.
• Working with others.
The position would suit an individual who enjoys working in a collaborative environment, manages their time effectively and is motivated by setting and reaching project milestones.
The MRC is a great place to work and progress your career, be it in scientific research or the support functions.
The MRC is a unique working environment where our researchers are rewarded by world class innovation and collaboration opportunities that the MRC name brings. The MRC is an excellent place to develop yourself further and a range of training & development opportunities will be available to you, including professional registration with the Science Council.
Choosing to come to work at the MRC (part of UKRI) means that you will have access to a whole host of benefits from a defined benefit pension scheme and excellent holiday entitlement to access to employee shopping/travel discounts and salary sacrifice cycle to work scheme, as well as the chance to put the MRC and UKRI on your CV in the future.
Our success is dependent upon our ability to embrace diversity and draw on the skills, understanding and experience of all our people. We welcome applications from all sections of the community irrespective of gender, race, ethnic or national origin, religion or belief, sexual orientation, disability or age. As "Disability Confident" employers, we guarantee to interview all applicants with disabilities who meet the minimum criteria for the vacancy.
Final appointments will be subject to pre-employment screening.