Leading science for better health

View Vacancy -- Research Support Officer - PNAC - Dr Leo James - LMB 934

Open Date
09/10/2018, 10:55
Close Date
11/11/2018, 23:55
MRC Laboratory of Molecular Biology

The MRC Laboratory of Molecular Biology (LMB) is one of the birthplaces of modern molecular biology and has played an important role in the development of many new techniques, most notably protein crystallography, cryo-electron microscopy, DNA sequencing and monoclonal antibodies.  The LMB has a clear goal of understanding biological processes at the molecular level, with the ultimate aim of using this knowledge to tackle specific problems in human health and disease.  We aim to tackle difficult, long-term problems, which often require investment over many years.

There are four scientific Divisions - Structural Studies, Protein and Nucleic Acid Chemistry, Cell Biology and Neurobiology.  However, scientific collaboration between the different Divisions is extensive.  Scientists are well supported by excellent scientific facilities and by the LMB Operations Group which maintains the core infrastructure and services of the institute.

The LMB provides an unsurpassed environment for both new and established researchers.  Scientists are drawn to the LMB from all over the world, thus creating a lively and international community for the exchange of ideas and technical innovation.

UK Research and Innovation is a new entity that brings together nine partners to create an independent organisation with a strong voice for research and innovation, more information can be found at www.ukri.org

MRC - 4/5
Cambridge
£22,201 - £33,305*
Fixed Term
Science
Full or Part Time options
Until 30 December 2021

A research support position is available in the James lab at the MRC Laboratory of Molecular Biology (LMB). We use multi-disciplinary approaches to address fundamental questions in health. You will join a highly integrated and motivated team with diverse skills and expertise across molecular biology. You will capitalise on our discovery of the cytosolic antibody receptor TRIM21 to help determine the molecular basis of its function and regulation. You will also help guide our ongoing development of ‘Trim-Away’ technology, which exploits TRIM21's unique ability to perform rapid and specific protein depletion. For more information on our work please see our website, recent published literature or contact (lcj@mrc-lmb.cam.ac.uk) for informal enquiries. Appointments will be made at either Band 4 Research Support Officer or Band 5 Research Support Assistant depending on experience. Please see below for full Job Descriptions and Person Specifications for the roles.

Band 4 Research Support Officer

* Starting salary £27,906 - £33,305, Band Salary £27,906 - £38,931

Overall purpose:

To work within the group of Dr Leo James at the Laboratory of Molecular Biology (LMB), providing research support for a programme investigating the immune response to viral infection.

To undertake independent research projects, supervise and manage other laboratory staff and provide training where appropriate.

Main duties

•    To design, perform and analyse a range of experiments that may include some but not necessarily all of:
     -    Design, reagent preparation, propagation and characterisation of human knockout cell lines using CRISPR/Cas9 technology.
     -    Propagation and characterization of stable cell lines.
     -    Construct generation for proteins of interest.
     -    Western blot analysis of proteins.
     -    Protein purification for antibody production.
     -    Screening for crystallisation conditions
     -    Biophysical measurements (eg ITC, SPR, ELISA)
     -    Cell culture assays (eg infection, qPCR, microscopy, immunoprecipitation)
•    To introduce and apply new techniques across a wide range of disciplines, and to have the creativity and initiative to develop novel approaches and methods where required.
•    Write up results of own research, analysing and interpreting results as appropriate, and generate original ideas based on outcomes.
•    To ensure the lab runs smoothly and efficiently; to manage day-to-day operations in the labs.
•    To keep up to date with developments in the field, proposing or implementing changes of direction as necessary.
•    To contribute to the preparation of research reports and publications.
•    To present information on research progress and outcomes as appropriate.
•    To contribute to the induction, training and direction of other research staff and students as requested by the Group Leader.
•    To ensure an adequate supply of lab consumables; to place orders for consumables and to follow up on orders as required.
•    To contribute to the overall activities of the research team by aiding in general lab duties.

Key responsibilities

To plan own work and objectives and manage experimental work within the project. There is scope for you to develop projects independently following discussion with Dr James.

To ensure the research is carried in accordance with good practice and in compliance with local polices and legal requirements.

To maintain awareness of health and safety regulations; to make sure all risk assessments are up-to-date and to ensure that all codes of practice are being followed.

To interact effectively with a wide range of staff, students and others to ensure the smooth running of the research group. This will include setting an example in the effective use of resources, training others in methods and use of equipment and taking responsibility for the use of communal facilities.

To ensure that equipment is safe and maintained in working order; arrange servicing and maintenance as necessary; liaise with other groups to arrange use of equipment held elsewhere.

To provide day-to-day supervision of students and training of postdocs in specific areas, as required by the Group Leader

Working relationships:

You will report to Dr Leo James and will liaise with other Research Support staff, post-doctoral scientists and students within the Group and across the LMB and with external collaborators as required.

Additional information:

This post is available either as a full time position but part time working will also be considered at a minimum of 3 days per week. Some flexibility in working hours might be required to keep cells over weekends.

The above lists are not exhaustive and you are required to undertake such duties as may reasonably be requested within the scope of the post. All employees are required to act professionally, co-operatively and flexibly in line with the requirements of the post and the MRC.


Band 5 Research Support Assistant

* Starting salary £22,201 - £25,976, Band Salary £22,201 - £29,837

Overall purpose:

To work within the group of Dr Leo James at the Laboratory of Molecular Biology (LMB), providing research support for a programme investigating the immune response to viral infection.

Main duties:

•    To undertake basic research by preparing, setting up, conducting and recording the outcome of experiments, analysing and interpreting results as appropriate.
•    To perform a range of experiments that may include some but not necessarily all of:
     -    Design, reagent preparation, propagation and characterization of human knockout cell lines using CRISPR/Cas9 technology.
     -    Propagation and characterization of stable cell lines.
     -    Construct generation for proteins of interest.
     -    Western blot analysis of proteins.
     -    Protein purification for antibody production.
     -    Screening for crystallisation conditions.
     -    Biophysical measurements (eg ITC, SPR, ELISA).
     -    Cell culture assays (eg infection, qPCR, microscopy, immunoprecipitation).
•    To present information on research progress and outcomes as appropriate.
•    To conduct literature and database searches.
•    To contribute to the production of research reports and publications.
•    To ensure and maintain an efficient and tidy Laboratory.

Key Responsibilities:

To plan and manage your experimental work within the project along with your supervisor and other members of the project team.

To ensure the research is carried in accordance with good practice and in compliance with local polices and legal requirements.

To contribute to the smooth running of the research group, including the effective use of resources, training of others and taking responsibility for use of communal facilities.

Working relationships:

You will report to Dr Leo James. You will interact and collaborate with Postdoctoral Scientists, Research Support Staff and Students, not only in your Group, but also across the LMB and with external Groups as necessary.

Additional information:
This post is ideally a full time position however part time working will also be considered at a minimum of 3 days per week. Some flexibility in working hours might be required to keep cells over weekends.

The above lists are not exhaustive and you are required to undertake such duties as may reasonably be requested within the scope of the post. All employees are required to act professionally, co-operatively and flexibly in line with the requirements of the post and the MRC.

Band 4 Research Support Officer

Education / qualifications / training required:

Essential: PhD in relevant biological/chemical subject area or the equivalent experience in a laboratory-based role and an undergraduate degree in a relevant biological/chemical subject.

Previous work experience required:

Essential:
•    Extensive experience using experimental techniques relevant to the role, which may include (though not limited to):
     -    Molecular biology techniques (eg PCR, gene cloning).
     -    Protein analysis: protein production and purification (affinity chromatography), gel electrophoresis, western blot.
     -    Cell culture.
     -    CRISPR/Cas9 gene targeting system.
     -    FACs and/or fluorescence microscopy.

•    Experience of planning experiments and successfully completing research projects, generating publications or patents.

Desirable:
•    Previous experience in the field of infection and immunity, viruses or similar.
•    Experience of purchasing equipment and consumables.

Knowledge and experience

Creativity and initiative
Essential:
•    Ability to identify, introduce and apply new techniques/methods across a range of molecular biological disciplines and to have the creativity and initiative to develop novel approaches as identified in consultation with the Group Leader.
•    Practical experience with running and maintaining scientific equipment.
•    Knowledge of laboratory health and safety.

Independent working
Essential:
•    Proven skills in working independently and as part of a team with minimal supervision, integrating different activities to ensure the successful delivery of the research projects.
•    Ability to problem solve using own initiative; good judgment in assessing when to ask for support.

Verbal and written communication skills
Essential:
•    Excellent written and verbal communication skills, with the ability to work collaboratively and harmoniously with colleagues of all cultures and backgrounds and as part of a team.
•    Ability to draft sections of scientific papers, and to contribute to the overall preparation of research for publication.

Desirable:
•    Experience of giving presentations.
•    Experience of providing day-to-day supervision (eg students, interns)
•    Evidence that scientific work has contributed to peer-reviewed publications.

Personal skills / behaviours / qualities:

Essential:
•    Focused, well organised and able to take initiative. With the flexibility to adapt to regularly changing priorities and workloads.
•    Reliable and enthusiastic with a passion for science and a desire to continue to develop skills and knowledge.


Band 5 Research Support Assistant

Education / qualifications / training required:

Essential: Bachelors degree or equivalent in a relevant biological subject.

Desirable: Relevant Masters or equivalent relevant laboratory experience.

Previous work experience required:

Essential:
•    Basic bench work skills.
•    Experience or theoretical knowledge of basic molecular biology skills/techniques (eg PCR, gene cloning).

Desirable:
•    Previous experience in the field of infection and immunity, viruses or similar.
•    Experimental techniques relevant to the role, which could include (though not limited to):
     -    Protein analysis: protein production and purification (affinity chromatography), gel electrophoresis, western blot.
     -    Cell culture.
     -    CRISPR/Cas9 gene targeting system.
     -    FACs and/or fluorescence microscopy experience.

Knowledge and Experience:

Creativity and initiative
Essential:
•    Ability to plan and carry out experiments, collate and analyse data.
•    Understanding of lab health and safety.

Desirable:
•    Practical experience of running and maintaining scientific equipment.

Independent working
Essential: Proven skills in working independently and as part of a team with minimal supervision; good judgment in assessing when to ask for support.

Desirable: Can independently advance existing techniques and implement new methods.

Verbal and written communication skills
Essential: Excellent written and verbal communication skills.

Desirable:
•    Experience of giving presentations.
•    Has made contributions to scientific papers.

Personal skills / behaviours / qualities:

Essential:
•    Reliable and enthusiastic.
•    Strong organisational skills.
•    Passion for science and a desire to continue to develop skills and knowledge.

This preferably a full time position (36 hours per week) however part time working will be considered for the right candidate  for a minimum of 3 days per week (21.6 hours per week).
The MRC is a great place to work and progress your career, be it in scientific research or the support functions.The MRC is a unique working environment where our researchers are rewarded by world class innovation and collaboration opportunities that the MRC name brings. The MRC is an excellent place to develop yourself further and a range of training & development opportunities will be available to you, including professional registration with the Science Council.

Choosing to come to work at the MRC (part of UKRI) means that you will have access to a whole host of benefits from a defined benefit pension scheme and excellent holiday entitlement to access to employee shopping/travel discounts and salary sacrifice cycle to work scheme, as well as the chance to put the MRC and UKRI on your CV in the future.

Our success is dependent upon our ability to embrace diversity and draw on the skills, understanding and experience of all our people. We welcome applications from all sections of the community irrespective of gender, race, ethnic or national origin, religion or belief, sexual orientation, disability or age. As "Disability Confident" employers, we guarantee to interview all applicants with disabilities who meet the minimum criteria for the vacancy.

UKRI supports research in areas that include animal health, agriculture and food security, and bioscience for health which includes research on animals, genetic modification and stem cell research. Whilst you may not have direct involvement in this type of research, you should consider whether this conflicts with your personal values or beliefs.

We will conduct a full and comprehensive pre-employment check as an essential part of the recruitment process on all individuals that are offered a position with UKRI. This will include a security check and an extreme organisations affiliation check.

This opportunity is closed to applications.